Project meeting of STEP at the site of the company ATS - Agro Trading & Solutions in Hardegsen (Photo: DBFZ)

Heat Power Biogas

Project ID 03KB120

Lasersize - Laser-based Back-reflection Analysis of the Particle Size Distribution for the Ultrasound-based Optimization of the Liquid Phase in Digestion Processes

Bio Cell Analyzer of Sequip in the fermenter (Photo: S + E Sequip GmbH)


  • 01.11.2016 – 31.12.2019


Technical University Berlin
Chair of Bioprocess Engineering
Ackerstraße 76
13355 Berlin

Prof. Peter Neubauer – project manager
Telephone: +49 (0)30 314 72573

Dr. Stefan Junne – project coordinator
Telephone: +49 (0)30 314 72527


03KB120B - S+E Sequip GmbH
  • Address:
  • S+E Sequip GmbH
    Angermunder Straße 22
    40489 Düsseldorf
  • Contact:
  • Friedel H. Schwartz – project manager
    Telephone: +49 (0)203 742140

03KB120C - Devad GmbH
  • Address:
  • Devad GmbH
    Oderstraße 53
    14513 Teltow
  • Contact:
  • Holger Hielscher – project manager
    Telephone: +49 (0)3328 437 4800

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Final report only available in German

Subject and aim

A novel method for the determination of the particle size distribution is adapted for the optimization of feedstock pre-treatment and exposure of the fluid phase of biogas processes to ultrasound. The solution shall be integrated easily in existing plants and the online measurement shall be analyzed and applied independently from other monitoring tools. The method will be applied in three industrial-scale case-studies.

Applying this approach, an increased substrate flexibility and operational efficiency is envisaged. Therefore, the ultrasound-based disruption of particles shall be monitored with laserlight-based back-reflection so that a real-time in situ optimization becomes feasible.


TU Berlin
Set up of a lab-scale experimental rig and application of the coupled disruption and analysis method in experimental series planned with statistical experimental design. Transfer into industrial scale so that the ultrasound- and analysis technique is applied in a bypass. An optimization of the ultrasound-based disruption based on lab experiments is conducted. Finally, an economic evaluation will be performed based on the gained data.

S+E Sequip GmbH
The hardware of sensors will be modified for their application for the measurement of the particle size distribution in the liquid phase of anaerobic digesters. Additionally, software will be modified to allow to distinguish between biologically active and inactive particles based on their back-reflection features.

Devad GmbH
Development and construction of a circulating ultrasound system, which includes the disruption of agglomerates and biomechanical material to optimize the liquid phase in digestion processes.


TU Berlin

  • Optimization of mechanical disruption methods
  • Conduction of industrial-scale case studies
  • Evaluation
  • Experimental design

S+E Sequip GmbH

  • Development of laser-based particle measurement probes
  • Analysis of particle detection
  • Adaptation to liquid matrices

Devad GmbH

  • Ultrasound-based disruption in different media
  • Scale-up
  • Systems integration


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